track acrylamide source from polyacrylamide gel that used in Hydraulic fracturing by stable isotopes method
write research proposal about (track acrylamide monomer contamination source from polyacrylamide gel that used in Hydraulic fracturing by stable isotopes method)
first mention what is polyacrylamid gel, where are used (e.g used genetic lab, hydraulic fracturing as friction agent, agriculture,etc,,), then describe how industry think it is safe and not consider a hazard, then describe the factors in environment that can lead to the degradation of polyaccrylamide gel from any kind of industry after that you will mention the health concern when this polyacrylamide gel in different industry can degraded to a toxic form which is acrylamide monomer, then describe the toxicity of the monomer, add many as you can find about accidental leaking or release of the polymer or it is monomer to environment and cause adverse effect to human and animals (e.g: the grout used in tunnel building cause sever illness to the labor because it has poly acrylamid gell in Sweden), find accident happened in hydraulic fracturing area all these introduction , now you will start the hypothetic question that How can we discriminate and track the origin of contamination , is the contamination caused by hydraulic fracturing or other industry , focus on that different acrylamide monomer can have different ration of stable isotope element such Carbon 13 or N , find litreture review articles describe the method of detection the presence of acrylamide in tested sample (hint: look for detection of acrylamid in food using (Liquid Chromatography Tandem Mass Spectroscopy) and find for me how can i apply stable isotopes to deferentiate between different origin of contamination, (please describe the method in proposal : how you can apply such testing method in steps and focus in apply QUALITY ASSURANCE in all procedure (from sampling : how to take samples, how many samples will be tested and why you choose this number of samples to test , what is best container to collect the field samples, and how to preserve it (important hint : you decide to collect soil sample from Hydraulic fracturing field to measure the acrylamid : don’t forgot to select the proper depth in the soil to take samples, what best condition to store the soil suspected to have acrylamide contamination , what perfect degree of storing and what is the container need depending in the chemical structure of the acrrylamid monomers) then the method of analysis on lab (you may used the method described previously in food ; how you will apply it in soil sample )
what is your QUALITY CONTROL (include REFERENCE sample for the polyacrylamide gel used specifically to HYDRAULIC FRACKING , include BLANKS, mention using delicate analysis for each sample to avoid mistakes in data) PLEASE, PLEASE ,PLEASE : DESCRIBE THE METHODOLOGY IN STEPS FORM NOT IN PARAGRAPH FORM.EACH STEP EXPLAIN WHY YOU DO IT. FOCUS IN PART OF ANALYSIS ON APLLYING STABL ISOTOPES METHOD TO TRACK THE HYDRAULIC FRACTURING SOURCE ( THINK AS A FORENSIC CHEMIST WANT TO DISCRIMINATE THE SOURCE OF CONTAMINATION). APPLY QUALITY ASSURANCE IN ALL THE PROCEDURE , LIKE (REFERENCE SAMPLE, BLANKS , PROPER STORING TO SAMPLE , FIND THE RANGE OF DETECTION LIMITS (HOW MUCH MY MACHINE CAN HIGHLY DETECT THE CONTAMINATION? look to EPA Organization website , see their protocol about sampling and analyzing the acrylamid , what their detection limit what is the Hazardous limit?
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